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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Pathogenicity to Mekong Giant Catfish Eggs of Water Moulds Isolated in the Laboratory from Mekong Giant Catfish Eggs and Rearing Water) ผู้เขียน: นายณรงค์ อาบกิ่ง ,  Wichukarn Fuangsawat, ดร.องอาจ เลาหวินิจ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractWater moulds in the genera Achlya and Saprolegnia were isolated from eggs of the Mekong giant catfi sh (Pangasianodon gigas) and from the water in the hatching tank at the Inland Aquaculture Research Institute, Phra Nakhon Sriayutthaya province, from 2008 to 2010. The optimal temperature of almost all isolates was 30 ?C. The Achlya spp. and Saprolegnia spp. could tolerate an NaCl medium at 10 and 25 parts per trillion (ppt), respectively. An exception was Saprolegnia sp. (E3/52-P2) which could tolerate NaCl up to 30 ppt. The isolates could grow in broth at pH 4–11, while the optimal pH for Achlya spp. and Saprolegnia spp. was pH 5 and pH6, respectively. The study on pathogenicity of the water moulds isolated in the laboratory showed that the isolates Achlya spp. (T.MCF1-02, E.MCF 2-001 and E4/52-10) and Saprolegnia sp. (E1/53-12) were pathogenic to the catfi sh eggs. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Sensitivity Comparison of Pathogenic Aquatic Fungal Hyphae to Sodium Chloride, Hydrogen Peroxide, Acetic Acid and Povidone Iodine) ผู้เขียน:Wichukarn Fuangsawat, นายณรงค์ อาบกิ่ง, ดร.องอาจ เลาหวินิจ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractNine isolates of pathogenic aquatic fungi: Aphanomyces piscicida NJM 9510, Aphanomyces astaci FDL 445, Aphanomyces sp. NJM 9406, Aphanomyces sp. NJM 9623, Saprolegnia diclina NJM 9219, Achlya sp. E. MCF 1-02, Achlya sp. T. MCF 1-02, Achlya bisexualis NJM 0611 and Achlya sp. NE 08, were tested for their sensitivity to four chemicals: sodium chloride, hydrogen peroxide, acetic acid and povidone iodine. Hyphal agents at the edge of the growing colonies were cut and immersed in various concentrations of the four chemicals for 60 min and then placed on glucose yeast extract agar. The results showed that the diameter of the fungal colonies increased when the chemical concentrations were decreased. The minimal inhibitory concentrations (MIC) of the nine isolates were: 5.00, 0.38, 0.75 and 0.31% for sodium chloride, hydrogen peroxide, acetic acid and povidone iodine, respectively. For the same four chemicals, the fungicidal concentrations of the nine isolates were: 20, 0.75, 1.60 and 0.63%, respectively. |
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